Influence of Media Composition on the Level of Bovine Satellite Cell Proliferation

It is predicted that already in 2040, 35% of requirements for meat will be provided by vitro production. Recreating the course myogenesis vitro, and thus resembling a structure muscle tissue, basis research focusing on obtaining cultured requires providing relevant factors supporting proliferation satellite cells—being precursors skeletal muscles. The present work aimed to develop composition medium would most effectively stimulate bovine cells (BSCs). modeling optimization methods included measurements synergistic, co-stimulatory effect three components: amount glucose, type serum (bovine or horse), mitogenic factor—bFGF. Additionally, qPCR analyses determined expression genes involved myogenesis, such as Pax7 Myogenic Regulatory Factors, depending level tested factor. results showed significant positive effects serum) factor (addition 10 ng/mL bFGF) rate. In turn, analysis displayed no differences relative MRF both factors. However, statistically higher Myf5 gene was revealed when low glucose used (p < 0.05). conclusion, components medium, addition at ng/mL, ensure rate BSCs lower content ensured crucial self-renewal cell population.